The infinitylab analytical hplc systems are highly efficient hplc and uhplc systems that feature the latest technology. Scope this sop is applicable for operation, handling, cleaning and maintenance of the high performance liquid chromatograph. Hplc or an agilent 1290 infinity uhplc system with this method. Sop for agilent 1100 series hplc instrument turn on all interface and wait until it reads not ready sop. Gas chromatographyheadspace gchs is a commonly used analytical technique in research and industrial laboratories for quality control as well as identification and quantitation of volatile compounds in a mixture. Quantitative determination of cannabinoids in hemp and hemp. This hplc calibration standard operating procedure sop describes all the individual steps. Procedure for calibration of hplc waters alliances. The optimum wavelength for a method can be found by acquiring the chromatographic data on a pda. We validate hplc methods, and we check every two weeks the mid point concentration of the calibration curve with a standard solution of the compound of our interest at a know concentration. Sample preparation ensure samples are free of particulate matter. Operation and calibration of agilent 1100 series gradient hplc.
This sop applies to agilent lcms qtof instrument located in the life sciences research building, room ls1151. Hplc calibration a complete guide part 1 of 3 pharma beginners. Water and sediment extracts samples were analyzed for extractable hydrocarbons by gas chromatographymass spectrometry gcms using an agilent agilent technologies, palo alto, ca, usa 7890 series gc and 5975 electron ionization ei mass selective. Agilent technologies 1200 series hplc standard operating.
Calibration cgmp hplc quality control sops 6 comments calibration of an instrument is the demonstration that the instrument or device produces results within specified limits by comparison with those produced by reference or traceable standard over an appropriate range of measurement. This is applicable to the operation of hplc system using chemistation software for agilent hplc system. Standard operating procedure for hplc calibration pdf. Standard operating procedure high performance liquid. Hplc, calibration, standard, operation, procedures gmp7. High performance liquid chromatography hplc youtube. Solvent phase transitioning it may be necessary to transition the hplc system from one solvent system to another.
Oct 22, 2019 for observation table refer sop for hplc calibration. How to make a calibration standard for an hplc sciencing. Risk assessment and sop for chemical wastes smb008 sop consultation, training and approval print names and enter signatures and dates to certify that the persons named in this section have. If not, you should prepare a calibration table before continuing with. Sop for agilent 1100 series hplc instrument turn on all interface and wait until it reads not ready agilent 1200 series hplc or an agilent 1290 infinity uhplc system with this method. Scope to ensure that the calibration procedure of hplc meets the acceptances criteria. Standard operating procedure for high performance liquid. Hplc calibration standard operation procedure youtube. To lay down the procedure for calibration of the hplc waters alliances 2695, separation modules. Standard operating procedure for hplc calibration pdf the sop for lipid analysis is in appendix c of the.
Agilent 1100 hplc chemstation oregon state university. However, the same principles may be applied to validations of other types of analytical procedures. Risk assessment and sop for toxic chemicals smb034 4. The 1220 infinity ii lc delivers high quality for an affordable price. Monoclonal antibody aggregate testing by hplc analysis.
Objective to lay down the procedure for calibration of the hplc waters alliances 2695, separation modules. Standard operating procedure remcho research group p a g e 1 agilent 1100 hplc chemstation 1 turn on all the modules for hplc system cappump g76a, dad g15b and allow for system to initialize. Dec 10, 2010 also one can archive standard operating procedures sops, specification and pharma jobs etc. This application note details a fast and simple analytical approach for quantitation of 9 cannabinoids of interest using an agilent 1260 infinity configured as an entry level instrument. This hplc calibration standard operating procedure sop describes all the individual steps necessary for calibrating a hplc in accordance with cgmp current good manufacturing practices. Agilent standard operating procedure analysis of emerging pharmaceutical contaminants by uhplcmsms.
Agilent 11001200 series g10a agilent 11001200 series g11b agilent 11001200 series g11a agilent 11001200 series g12a agilent 11001200 series g12b pump module of the waters alliance 2690 separation module tsp p2000 tsp p4000 shimadzu lc2010 pump shimadzu lc10atvp shimadzu lc10advp. Dec 23, 2012 this hplc calibration standard operating procedure sop describes all the individual steps necessary for calibrating a hplc in accordance with cgmp current good manufacturing practices. We keep the calibration curve if the results is within the 5% of the expected concentration. These instructions assume that there is a calibration table prepared for the method. Wellcharacterised reference materials with documented purity should be used to perform the validation. Inject duplicate injections of 5 ppm, 20 ppm, 50 ppm and 100 ppm of caffeine solution mentioned in injector precision test. Hplc calibration a complete guide part 1 of 3 pharma. This sop uses an hplc to quantify the caffeine concentration in generic brewed coffee by assaying a series of caffeine standards in. Biosystems engineering hplc standard operating procedure. Fast and easy cannabis potency testing using an entry level. Buck scientific blc20p hplc system preconfigured with. The 1290 infinity ii lc embodies the next generation of liquid. Reston biogeochemical processes in groundwater laboratory. Calibration, gc, hplc, uvvisible spectrophotometer, validation.
When working with high performance liquid chromatography hplc, good calibration is absolutely essential to ensure reliable, quality results. Feb 20, 2017 high performance liquid chromatography hplc. To ensure that the calibration procedure of hplc meets the acceptances criteria. In most instances, calibration in fact requires a series of standards of. This hplc calibration standard operating procedure sop describes all the individual steps necessary for calibrating a hplc in. This method is designed to allow a user to process organic base screen, organic base quant, acid and neutral screen, acid and neutral quant, and lc screen data and create reports from data acquired by an agilent gc npd, fid andor. Validation and calibration of analytical instruments. Ensure that, the instrument is ready for calibration and startup procedure is followed. See the advion users manual if you need to create a custom calibration for your analysis. Jul 03, 2019 set up the hplc system using chromatographic conditions as given below. Analyses of high pressure liquid chromatography hplc calibration standards cs, method blanks. Quantitative determination of cannabinoids in hemp and hemp products by high performance liquid chromatography using a uv detector 1. Proper calibration of an hplc instrument begins with the making of a suitable calibration standard. Pull down the calibration menu and select calibraterecalibrate 2.
As for any calibration in the pharmaceutical environment, the calibration of the hplc also needs to be described in a procedure and followed by the analyst in line with regulations. Standard operating procedure high performance liquid chromatography hplc agilent technologies 1260 infinity high performance liquid chromatography hplc is a technique in analytical chemistry used to separate, identify, and quantify each component in a liquid mixture. The use of dynamic mrm greatly improved this process of. Similarly, all transitions in this sop will perform well. An excel program lindas hplc standard sugar solution makeup has the makeup and calculates the amounts needed to make up 6 calibration standards. The agilent 6530b accurate mass quadruple timeofflight mass spectrometer is equipped with dual electrospray ionization source and an agilent 1260 uplc liquid. The purity of the buffer and flush water is critical do not skip the filtration step. Mobile phase preparation mix 700 ml of hplc grade water and 300 ml of methanol and filter through 0. It is the responsibility of the studentstechnician to follow the sop as described and to inform the instructor about any deviations or problems that may occur while performing the procedure. To define the precautions to be taken during handling. Agilent 1100 hplc chemstation 1 turn on all the modules for hplc system cappump g76a, dad g15b and allow for system to initialize.
Validation of an analytical procedure is the process by which it is established, by laboratory studies, that the performance characteristics of the procedure meet the requirements for the intended analytical applications. High performance liquid chromatography hplc is an analytical chemistry technique for separating the components of a liquid sample and for identifying and quantifying the components of the sample. Store filtered buffer into an appropriate container 1, 2 or 4l glass bottle with 33mm threaded top 6. Oct 04, 2015 validation and calibration of hplc 1 by sakshi garg m. Friday, december 10, 2010 operation and calibration of hplc system simadzu. Operational qualification performance qualification for hplc. Similarly, all transitions in this sop will perform well with the agilent 6410 and 6430 triple quadrupole lc systems. Hplc calibration procedure know the procedure to calibrate the high performance liquid chromatography hplc including leakage test, flow rate, reproducibility and linearity, lamp energy and pump pressure drop in pharmaceutical quality control. Manual method recalibration procedure gc, lc ce and ad chemstation, revision a. Sop for hplc system using chemistation software from agilent. Select average or replace in the recalibration section this document is believed to be accurate and uptodate. The 1260 infinity ii lc is the flexible instrument choice, while the 1260 infinity ii prime lc brings more operational convenience.
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